Frank Welker

as Scooby-Doo / Rudy / Fred / Nefario (voice)

Grey DeLisle

as Daphne / Daisy / Musketeer 1 / Olive (voice)

Matthew Lillard

as Shaggy / Craggly / Captain Cutler (voice)

Kate Micucci

as Velma / Helga (voice)

Myrna Velasco

as Coco Diablo (voice)

Dee Bradley Baker

as Esteban / Mr. Wickles / Cat Man (voice)

Jeff Bennett

as Shady Guy / Charlie Humdrum / Hank (voice)

Anthony Carrigan

as Trevor Glume / Farmer (voice)