Timothy Farrell

as Dr. Alton / Narrator

Bela Lugosi

as Scientist

Edward D. Wood Jr.

as Glen / Glenda (as Daniel Davis)

Lyle Talbot

as Inspector Warren

Dolores Fuller

as Barbara

'Tommy' Haynes

as Alan / Anne

Charlie Crafts

as Johnny (as Charles Crafts)

Conrad Brooks

as Banker / Reporter / Pickup Artist / Bearded Drag (as Connie Brooks)