Nova Pilbeam

as Lady Jane Grey

Cedric Hardwicke

as Earl of Warwick

John Mills

as Lord Guilford Dudley

Felix Aylmer

as Edward Seymour

Leslie Perrins

as Thomas Seymour

Frank Cellier

as Henry VIII

Desmond Tester

as Edward VI

Gwen Ffrangcon Davies

as Mary Tudor (as Gwen Ffrangcon-Davies)