Cedric Hardwicke

as Dr. Ludwig Frankenstein / Dr. Henry Frankenstein (ghostly image)

Ralph Bellamy

as Erik Ernst

Lionel Atwill

as Dr. Bohmer

Bela Lugosi

as Ygor

Evelyn Ankers

as Elsa Frankenstein

Lon Chaney Jr.

as The Frankenstein Monster

Janet Ann Gallow

as Cloestine Hussman

Barton Yarborough

as Dr. Kettering