Magda Vášáryová

as Mary Stuart

Joan Sutherland

as Mary Stuart (singing voice)

Kamila Magálová

as Queen Elizabeth I

Huguette Tourangeau

as Queen Elizabeth I (singing voice)

Milan Kňažko

as Earl of Leicester

Luciano Pavarotti

as Earl of Leicester (singing voice)

Přemysl Kočí

as George Talbot

Roger Soyer

as George Talbot (singing voice)