Dakota Fanning

as Fern Arable

Julia Roberts

as Charlotte the Spider (voice)

Steve Buscemi

as Templeton the Rat (voice)

John Cleese

as Samuel the Sheep (voice)

Oprah Winfrey

as Gussy the Goose (voice)

Cedric the Entertainer

as Golly the Goose (voice)

Reba McEntire

as Betsy the Cow (voice)

Kathy Bates

as Bitsy the Cow (voice)